First report and molecular confirmation of Potyvirus phaseovulgaris associated with yellow mosaic on cowpea Vigna unguiculata in Iraq
Keywords:
yellow mosaic, phylogeny, legumesAbstract
Potyvirus phaseovulgaris also known as Bean common mosaic virus (BCMV) was investigated in cowpea samples collected from a field located at Al-Jadriya/Baghdad. Sampling included leaves collected from symptomatic plants (n=16). RNA was extracted from leaf samples and exposed to cDNA synthesis. The presence of BCMV was detected using Nested Revers Transcriptase Polymerase Chain Reaction (N-RT-PCR) and species-specific primer sets. Gel electrophoresis pattern revealed, nested primers designed could targeted BCMV when the resultant expected size DNA fragments of ~500 bp were amplified from 2 out of 16 samples only indicating they could be BCMV infected. Nucleotide sequence comparison confirmed BCMV detection in samples when sequences of 500 bp DNA fragments amplified shared maximum 98% nucleotide identity with the equivalent sequence of a Chinese isolate (MN786956) retrieved from the National Center for Biotechnology Information NCBI data base. Our data suggests the first confirmation of BCMV on cowpea in Iraq based on molecular approaches
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